RBS
Part:BBa_J100458:Design
Designed by: Eleanor McCall Group: Campbell_M_Lab (2018-10-23)
Even More As
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Using trial and error in mFold software, we used the energy dot plot to select an unstable sequence to prevent the sequence from folding and becoming unable to bind to the ribosome.
Source
We studied results found in a similar experiment in "rClone Red Facilitates Bacterial Gene Expression Research by Undergraduates in the Teaching Laboratory" by A. Malcolm Campbell and Todd T. Eckdahl. http://bio.davidson.edu/113/113labscedule2018.html We determined stability of sequence using this mFold web server: RNA Folding Form. (n.d.). Retrieved October 16, 2018, from The RNA Institute - College of Arts and Sciences - University at Albany website: http://unafold.rna.albany.edu/?q=mfold/RNA-Folding-Form