RBS

Part:BBa_J100458:Design

Designed by: Eleanor McCall   Group: Campbell_M_Lab   (2018-10-23)


Even More As


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Using trial and error in mFold software, we used the energy dot plot to select an unstable sequence to prevent the sequence from folding and becoming unable to bind to the ribosome.


Source

We studied results found in a similar experiment in "rClone Red Facilitates Bacterial Gene Expression Research by Undergraduates in the Teaching Laboratory" by A. Malcolm Campbell and Todd T. Eckdahl. http://bio.davidson.edu/113/113labscedule2018.html We determined stability of sequence using this mFold web server: RNA Folding Form. (n.d.). Retrieved October 16, 2018, from The RNA Institute - College of Arts and Sciences - University at Albany website: http://unafold.rna.albany.edu/?q=mfold/RNA-Folding-Form

References